Waters MS Technology Researchers Bob Bateman and
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The novel MS acquisition mode was announced at the 15th
In modern proteomics experiments, DIA-based mass spectrometry has been a popular technique for capturing data-rich sample profiles. As proteomics and lipidomics capabilities have progressed, scientists seek to further focus experiments to quantify specific peptides and proteins, which has required additional method development and repeated assays. The newly introduced SONAR data acquisition mode provides additional clarity and information for increasingly complex samples.
“Today, as proteomics research has matured, scientists are already
collecting the majority of the information available from proteins. What
they want to be able to do is approach a protein or specific peptides
with a hypothesis, and use targeted MS/MS quantification to investigate
their ideas without setting up additional methods or experiments,” said
Waters Scientists Recognized at HUPO
Also at the annual HUPO meeting, Waters Corporation’s Consultant for
“The availability of a Q-Tof tandem MS instrument had tremendous influence on the development of proteomics in its early days, since this type of mass spectrometer coupled with a nanoLC provided unprecedented performance in analyzing a proteome,” noted the HUPO Executive Committee in their award announcement. The Waters® (Micromass®) Q-Tof™ Mass Spectrometer was first commercially introduced in 1996 and has undergone many technological advancements, most recently with the integration of ion mobility separations and now with its novel SONAR MS data acquisition mode.
SONAR Enhancements to MS Data Acquisition Modes
The additional selectivity offered by the SONAR data acquisition mode is due to the behavior of the mass spectrometer’s quadrupole. Instead of remaining open and transmitting all ions, in SONAR mode the quadrupole slides over the selected mass range of interest, with each scan capturing 200 spectra. The operation of the quadrupole makes the SONAR mode compatible with fast UltraPerformance LC® (UPLC®) separations that increase laboratory throughput. Compounds that may have chromatographically coeluted are now separated by the quadrupole and recorded individually for more effective library searches. The SONAR data acquisition mode collects both quantitative and qualitative data within a single injection.
SONAR technology is the subject of several
SONAR data is compatible with analytical workflows that utilize Waters Progenesis® and Symphony™ software, and with third-party software packages such as Skyline. SONAR is available on the Waters Xevo G2-XS QTof Mass Spectrometer under MassLynx® Software control.
Waters, SONAR, Xevo, Micromass, Q-Tof, UltraPerformance LC, UPLC,
Progenesis, Symphony, and MassLynx are trademarks of
Karen Haas, +1-508-482-3363