ACQUITY UltraPerformance Liquid Chromatography Paired With Xevo TQ and Synapt HDMS Mass Spectrometers Target Small Molecule Biomarkers Linked to Disease
Identifying and quantifying the thousands of metabolic biomarkers present in a complex sample such as urine can be a daunting task. Only with the most sensitive of instruments and most powerful of software can researchers uncover and pinpoint the important biomarkers that may be present even in the smallest of quantities. This level of advanced research requires analytical tools with exceptional separations and confirmatory power, and the speed and reliability to analyze thousands of samples per month. Since purchasing an ACQUITY UPLC/Q-Tof Premier(TM) mass spectrometer in 2005, the Center has tested 80,000 samples using this system in support of various research studies.
Waters ACQUITY UPLC System employs 1.7 micron particles in analytical columns that permit higher chromatographic resolution, shorter analysis times, and the use of less solvent per sample. Paired with tandem quadrupole and quadrupole time-of-flight/ion mobility mass spectrometry, the sharper chromatographic peaks, increased peak heights, and better signal-to-noise performance of UPLC(R) technology improves the mass spectrometer's ability to ionize molecules. This capability leads to greater all-around MS sensitivity and the ability to accurately identify vanishingly small concentrations of individual analytes. UPLC separations typically take only 10 minutes versus nearly 50 minutes for traditional HPLC to get the same answer leading to dramatically better throughput. UPLC consumes up to 95 percent less solvent than HPLC, a major consideration given the current shortage and rising cost of acetonitrile and the amount of solvent that metabolomics studies consume.
Waters Xevo TQ MS System is an advanced tandem quadrupole mass spectrometer that tackles the most demanding quantification applications for target compounds at low concentrations in highly complex and diverse samples. The Xevo TQ MS System uses ScanWave(TM), a unique collision cell technology based on a novel use of Waters' proven T-Wave(TM) technology that improves duty cycle and significantly enhances the full scan capability to meet the demands of today's complex analyses. This allows scientists to more easily confirm the identities and structures of targeted analytes.
The Synapt(TM) HDMS system combines quadrupole time-of-flight with ion-mobility technology and software to enable the analysis of sample ions differentiated by size and shape, as well as mass. This additional dimension of separations fidelity leads to improved specificity and sample definition, meaning scientists can extract more information about their samples and detect previously unseen constituents.
Together with bioinformatics software tools incorporated in Waters MassLynx(TM) software, researchers using UPLC/MS/MS can analyze tens of thousands of data points per analysis, ultimately creating a more informed picture of the mechanisms of toxicity and carcinogenicity in human patients.
Pioneering a connected portfolio of separations science, laboratory information management, mass spectrometry and thermal analysis, Waters technology breakthroughs and laboratory solutions provide an enduring platform for customer success.
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Waters, Synapt, MassLynx, ACQUITY, ACQUITY UPLC, ACQUITY UltraPerformance
LC, UPLC, High Definition Mass Spectrometry, High Definition MS, Q-Tof
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